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KMID : 0357919800140030015
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Korean Journal of Pathology
1980 Volume.14 No. 3 p.15 ~ p.20
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Early Detection of Mycobacterium tuberculosis in Sputum by Slide Culture
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이원길(李源吉)/Won Kil Lee
김재식(金在植)/김중명(金重明)/Jae Sik Kim/Jyung Myung Kim
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Abstract
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An early detection of Mycobacterium tuberculosis in the sputum was attempted by slide
culture method instead of the ordinary culture media and the authors obtained the
following results.
The elongation of bacterial cells was only observed on the slide of the first day culture.
There was increases of the number of the elongated cells on the third day culture.
There were many groups which composed of the elongated cells of mycobcteria
containing granules in the cells on the slide of the fifth day culture. On the seventy day
culture the microcolony formation of the elongated cells was prominent. Also the
elongated cells, the cells with granules and the divided cells were seen scattered.
The rate of the culture positivity from 102 sputums was 9.8% on the Ogawa's media
and 10.8% on the Kirchner's media. The rate of positivity of slide culture method was
the same as the culture on the Kirchner media.
The rate of positivity by the direct smear staining of the sputum was 9.8%. The
microscopically positive 2 cases were negative in the cultures while the microscopically
negative 3 cases were positive in the culture.
The slice culture method was thought to be useful for the early detection of the
Mycobacterium tuberculosis in the sputum.
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